Combining Affinity Selection and Specific Ion Mobility for Microchip Protein Sensing

Analysis of site-specific protein-RNA cross-links in isolated RNP complexes, combining affinity selection and mass spectrometry.

An important aspect of the assembly of RNPs, and in particular of spliceosomes, is the succession of proteins bound to any given site on the RNA. Protein-RNA cross-linking is a well-established technique for investigating this, but the identification of a cross-linked protein has so far relied upon the availability of antibodies for immunoprecipitation or Western blot studies. To facilitate ide...

selection of high affinity dna-aptamer for activated protein c using capillary electrophoresis

Advancements in capillary-assembled microchip (CAs-CHIP) development for multiple analyte sensing and microchip electrophoresis.

This review describes advancements toward the development of a capillary-assembled microchip (CAs-CHIP) for simultaneous multiple analyte sensing and microchip capillary electrophoresis. Development of such an advanced system relies on several factors such as improving the fluid handling technique, creating new biosensing mechanisms, and integrating different functional capillaries into a singl...

Combining feature selection and feature reduction for protein classification

We use the n-grams descriptors for a protein classification task. As they are automatically generated, we obtain many irrelevant and/or redundant descriptors. In this paper, we evaluate various strategies of feature selection and feature reduction. First, we evaluate separately the efficiency of a filtering feature selection algorithm and a feature reduction on the basis of a singular value dec...

Direct Dynamic Protein-Affinity Selection Mass-Spectrometry

A new methodology is described enabling the affinity screening of potential ligands towards the human estrogen receptor alpha ligand binding domain (ERalpha-LBD). In-solution incubation is performed of the analyte and the His-tagged ERalpha-LBD. The bound complex is immobilized on a nickel-loaded protein-affinity selection column, where after the unbound fraction is removed. The immobilized pro...